red 637 nm wavelength thorlabs laser diode Search Results


laser  (Thorlabs)
86
Thorlabs laser
Laser, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser diode  (Thorlabs)
86
Thorlabs laser diode
Laser Diode, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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continuous wave laser beam  (Thorlabs)
86
Thorlabs continuous wave laser beam
Continuous Wave Laser Beam, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser stimulation  (Thorlabs)
86
Thorlabs laser stimulation
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Laser Stimulation, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/laser+stimulation/bio_rxiv__2024__08__14__607152-98-12-16
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pump laser  (Thorlabs)
86
Thorlabs pump laser
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Pump Laser, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/laser+pump/arxiv__2411__16830-138-7-18
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lasers  (Thorlabs)
86
Thorlabs lasers
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Lasers, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/lasers/arxiv__2411__17187-223-21-34
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fiber coupled semiconductor laser  (Thorlabs)
86
Thorlabs fiber coupled semiconductor laser
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Fiber Coupled Semiconductor Laser, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/coupled+fiber+led/10__1364_slash_ol__41__004146-26-5-8
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s4fc637  (Thorlabs)
86
Thorlabs s4fc637
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
S4fc637, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/s4fc637/pmc12857275-46-7-5
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variable fiberoptic attenuators  (Thorlabs)
86
Thorlabs variable fiberoptic attenuators
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Variable Fiberoptic Attenuators, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/attenuators+optical+variable/arxiv__2307__07198-171-26-35
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thorlabs f810apc 635  (Thorlabs)
86
Thorlabs thorlabs f810apc 635
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Thorlabs F810apc 635, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/780+f810apc/pmc12717926-217-15-15
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filter  (Thorlabs)
86
Thorlabs filter
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Filter, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/red+637+nm+wavelength+thorlabs+laser+diode/filter/pmc12717926-217-23-24
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filter - by Bioz Stars, 2026-10
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thorlabs ldm785 laser  (Thorlabs)
86
Thorlabs thorlabs ldm785 laser
(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser <t>stimulation</t> and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).
Thorlabs Ldm785 Laser, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser stimulation and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).

Journal: bioRxiv

Article Title: Overcoming off-target optical stimulation-evoked cortical activity in the mouse brain in vivo

doi: 10.1101/2024.08.14.607152

Figure Lengend Snippet: (A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed in the contralateral VIS in complete darkness. Black dental cement in and around the headplate and blackout adhesive around the optical fiber were used to prevent light emitting through the craniotomy, skull and optical fiber. In addition, eye cups were gently positioned to touch the fur around the eye to further ensure there to be no external activation of the retina. (B) Raster plots of spikes of three individual units in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser stimulation and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed in complete darkness with laser power of 15 or 1mW. Bar plots display the average percentage of the total number of units (n = 761) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using either 15mW (top) or 1mW (bottom). Grey shaded area indicates sem. (D) Average difference in firing rates (mean ± sem) between a baseline window (0.2 s before stimulation) and a response window (0.5 s after onset of stimulation) for red, orange or blue laser stimulation at different laser power. Asterisks indicate significant differences between red and blue (black) as well as red and orange (gray). (E) Violin plots displaying onset latencies of red, orange and blue laser-evoked responses at 15mW (top) and 1mW (bottom) laser stimulation. Individual data points (circles), median (black circle) and first and third quartiles are displayed (black lines).

Article Snippet: An optical cord (Newdoon MM200/220 NA 0.37) was used to provide the laser stimulation (637 nm: Thorlabs S4FC637; 594 nm: Coherent OBIS 594nm LS 60mW; 473nm: Coherent OBIS 473nm LX 150mW).

Techniques: Adhesive, Activation Assay

(A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed on the contralateral VIS. External illumination was delivered via two monitors displaying a full screen iso-illuminant gray image. (B) Raster plots of spikes of three individual units (same cells as in ) in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser stimulation and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed either in complete darkness with laser power of 15 or 1mW or under ambient light conditions (indicated by the grey bar). Bar plots display the average percentage of the total number of units (n = 761 units, n = 4 mice) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW under darkness or ambient light conditions. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Scatter plot displaying the average laser-evoked firing rate change for those cells that responded under darkness versus ambient light conditions for blue (n = 184), orange (n = 249), red (n = 265) laser stimulations at 15mW. (D) Heatmaps displaying the percentage of units modulated by blue (left), orange (middle) and red (right) laser stimulation under dark (top row) and different ambient light conditions and laser powers. Bottom row displays the false positive percentage for pooled dark and ambient light conditions according to the ZETA test used to assess significant modulation during a control period when laser stimulation was absent. Asterisks indicate significant differences between percentages of modulated units under stimulation periods (rows 1-4) compared to baseline periods without any laser stimulation (row 5). (E) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using 20 lux of ambient light brightness and 15mW laser stimulation intensity. Grey shaded area indicates sem. (F) Same as E for 2.5mW laser stimulation intensity.

Journal: bioRxiv

Article Title: Overcoming off-target optical stimulation-evoked cortical activity in the mouse brain in vivo

doi: 10.1101/2024.08.14.607152

Figure Lengend Snippet: (A) Schematic of the recording setup. Red (673 nm), orange (594 nm) or blue (473 nm) laser was delivered via an optical fiber implanted in the visual cortex (VIS, left hemisphere) of awake head-fixed mice. Translaminar Neuropixels recordings were performed on the contralateral VIS. External illumination was delivered via two monitors displaying a full screen iso-illuminant gray image. (B) Raster plots of spikes of three individual units (same cells as in ) in response to ten repetitions of 0.5 s of blue (left), orange (middle) or red (right) laser stimulation and the corresponding average peri-stimulus time histogram (PSTH). Recordings were performed either in complete darkness with laser power of 15 or 1mW or under ambient light conditions (indicated by the grey bar). Bar plots display the average percentage of the total number of units (n = 761 units, n = 4 mice) activated by blue, orange and red laser stimulation across 1, 2.5, 5, 10 and 15mW under darkness or ambient light conditions. Open circles indicate the percentage of responding units for each laser intensity. Error bars represent the sem of the percentage of responders over the five laser intensities. (C) Scatter plot displaying the average laser-evoked firing rate change for those cells that responded under darkness versus ambient light conditions for blue (n = 184), orange (n = 249), red (n = 265) laser stimulations at 15mW. (D) Heatmaps displaying the percentage of units modulated by blue (left), orange (middle) and red (right) laser stimulation under dark (top row) and different ambient light conditions and laser powers. Bottom row displays the false positive percentage for pooled dark and ambient light conditions according to the ZETA test used to assess significant modulation during a control period when laser stimulation was absent. Asterisks indicate significant differences between percentages of modulated units under stimulation periods (rows 1-4) compared to baseline periods without any laser stimulation (row 5). (E) Average spike density functions for all units aligned to the onset (dotted line) of red, orange or blue laser stimulation (n = 761 cells, n = 4 mice) using 20 lux of ambient light brightness and 15mW laser stimulation intensity. Grey shaded area indicates sem. (F) Same as E for 2.5mW laser stimulation intensity.

Article Snippet: An optical cord (Newdoon MM200/220 NA 0.37) was used to provide the laser stimulation (637 nm: Thorlabs S4FC637; 594 nm: Coherent OBIS 594nm LS 60mW; 473nm: Coherent OBIS 473nm LX 150mW).

Techniques: Control